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diffuse reflectance standard usrs-99-020  (Labsphere Inc)

 
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    Labsphere Inc diffuse reflectance standard usrs-99-020
    Schematic of the combined SFDI and fluorescence imaging system for aPDT dosimetry. (a) Projection arm of the system provides spatially modulated light at four different wavelengths (395, 545, 625, and 850 nm) using two independent amplitude masks. The light is spatially combined and passed through a removable linear polarizer before being projected onto the sample, indicated with an orange arrow, at an angle. (b) Separately, the treatment arm delivers the light to the sample for PDT treatment and fluorescence excitation. The resulting <t>reflectance</t> and fluorescence signals are collected by the imaging arm at three different channels, λ < 590 nm , 590 nm ≤ λ ≤ 660 nm , and λ > 660 , where the middle channel is further cleaned up with a bandpass filter. (c) Picture of the combined imaging system. (d) Pictures of the printed amplitude masks used for 0.3 mm − 1 (left) and 1.0 mm − 1 (right) patterned illumination. (e) Normalized intensity profiles of the four projection LEDs at their respective detectors. ACL, aspheric condenser lens; DM, dichroic mirror; LP, linear polarizer; AD, achromatic doublet; BPF, bandpass filter; HAT, Hastings achromatic triplet.
    Diffuse Reflectance Standard Usrs 99 020, supplied by Labsphere Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diffuse reflectance standard usrs-99-020/product/Labsphere Inc
    Average 90 stars, based on 1 article reviews
    diffuse reflectance standard usrs-99-020 - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "Development and characterization of a combined fluorescence and spatial frequency domain imaging system for real-time dosimetry of photodynamic therapy"

    Article Title: Development and characterization of a combined fluorescence and spatial frequency domain imaging system for real-time dosimetry of photodynamic therapy

    Journal: Journal of Biomedical Optics

    doi: 10.1117/1.JBO.30.S3.S34103

    Schematic of the combined SFDI and fluorescence imaging system for aPDT dosimetry. (a) Projection arm of the system provides spatially modulated light at four different wavelengths (395, 545, 625, and 850 nm) using two independent amplitude masks. The light is spatially combined and passed through a removable linear polarizer before being projected onto the sample, indicated with an orange arrow, at an angle. (b) Separately, the treatment arm delivers the light to the sample for PDT treatment and fluorescence excitation. The resulting reflectance and fluorescence signals are collected by the imaging arm at three different channels, λ < 590 nm , 590 nm ≤ λ ≤ 660 nm , and λ > 660 , where the middle channel is further cleaned up with a bandpass filter. (c) Picture of the combined imaging system. (d) Pictures of the printed amplitude masks used for 0.3 mm − 1 (left) and 1.0 mm − 1 (right) patterned illumination. (e) Normalized intensity profiles of the four projection LEDs at their respective detectors. ACL, aspheric condenser lens; DM, dichroic mirror; LP, linear polarizer; AD, achromatic doublet; BPF, bandpass filter; HAT, Hastings achromatic triplet.
    Figure Legend Snippet: Schematic of the combined SFDI and fluorescence imaging system for aPDT dosimetry. (a) Projection arm of the system provides spatially modulated light at four different wavelengths (395, 545, 625, and 850 nm) using two independent amplitude masks. The light is spatially combined and passed through a removable linear polarizer before being projected onto the sample, indicated with an orange arrow, at an angle. (b) Separately, the treatment arm delivers the light to the sample for PDT treatment and fluorescence excitation. The resulting reflectance and fluorescence signals are collected by the imaging arm at three different channels, λ < 590 nm , 590 nm ≤ λ ≤ 660 nm , and λ > 660 , where the middle channel is further cleaned up with a bandpass filter. (c) Picture of the combined imaging system. (d) Pictures of the printed amplitude masks used for 0.3 mm − 1 (left) and 1.0 mm − 1 (right) patterned illumination. (e) Normalized intensity profiles of the four projection LEDs at their respective detectors. ACL, aspheric condenser lens; DM, dichroic mirror; LP, linear polarizer; AD, achromatic doublet; BPF, bandpass filter; HAT, Hastings achromatic triplet.

    Techniques Used: Fluorescence, Imaging

    Predicted spatial frequency–dependent reflectance of the human skin. SFDI Monte Carlo simulations were performed using a seven-layer human skin model (a) with a fixed width (1 mm) and depth (3 mm) and a variable length such that two full periods of the patterned illumination were always applied. The thickness ( d , mm), scattering anisotropy ( g ), and refractive index ( n ) for each layer were fixed, whereas the absorption ( μ a , mm − 1 ) and reduced scattering ( μ s ′ , mm − 1 ) coefficients were varied for the four illumination wavelengths. The absolute (b) and normalized (c) Monte Carlo results for the reflectance of the seven-layer model with spatial frequencies between 0 and 1.0 mm − 1 at the four illumination wavelengths.
    Figure Legend Snippet: Predicted spatial frequency–dependent reflectance of the human skin. SFDI Monte Carlo simulations were performed using a seven-layer human skin model (a) with a fixed width (1 mm) and depth (3 mm) and a variable length such that two full periods of the patterned illumination were always applied. The thickness ( d , mm), scattering anisotropy ( g ), and refractive index ( n ) for each layer were fixed, whereas the absorption ( μ a , mm − 1 ) and reduced scattering ( μ s ′ , mm − 1 ) coefficients were varied for the four illumination wavelengths. The absolute (b) and normalized (c) Monte Carlo results for the reflectance of the seven-layer model with spatial frequencies between 0 and 1.0 mm − 1 at the four illumination wavelengths.

    Techniques Used: Refractive Index



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    Schematic of the combined SFDI and fluorescence imaging system for aPDT dosimetry. (a) Projection arm of the system provides spatially modulated light at four different wavelengths (395, 545, 625, and 850 nm) using two independent amplitude masks. The light is spatially combined and passed through a removable linear polarizer before being projected onto the sample, indicated with an orange arrow, at an angle. (b) Separately, the treatment arm delivers the light to the sample for PDT treatment and fluorescence excitation. The resulting <t>reflectance</t> and fluorescence signals are collected by the imaging arm at three different channels, λ < 590 nm , 590 nm ≤ λ ≤ 660 nm , and λ > 660 , where the middle channel is further cleaned up with a bandpass filter. (c) Picture of the combined imaging system. (d) Pictures of the printed amplitude masks used for 0.3 mm − 1 (left) and 1.0 mm − 1 (right) patterned illumination. (e) Normalized intensity profiles of the four projection LEDs at their respective detectors. ACL, aspheric condenser lens; DM, dichroic mirror; LP, linear polarizer; AD, achromatic doublet; BPF, bandpass filter; HAT, Hastings achromatic triplet.
    Diffuse Reflectance Standard Usrs 99 020, supplied by Labsphere Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diffuse reflectance standard usrs-99-020/product/Labsphere Inc
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    diffuse reflectance standard usrs-99-020 - by Bioz Stars, 2026-06
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    Schematic of the combined SFDI and fluorescence imaging system for aPDT dosimetry. (a) Projection arm of the system provides spatially modulated light at four different wavelengths (395, 545, 625, and 850 nm) using two independent amplitude masks. The light is spatially combined and passed through a removable linear polarizer before being projected onto the sample, indicated with an orange arrow, at an angle. (b) Separately, the treatment arm delivers the light to the sample for PDT treatment and fluorescence excitation. The resulting <t>reflectance</t> and fluorescence signals are collected by the imaging arm at three different channels, λ < 590 nm , 590 nm ≤ λ ≤ 660 nm , and λ > 660 , where the middle channel is further cleaned up with a bandpass filter. (c) Picture of the combined imaging system. (d) Pictures of the printed amplitude masks used for 0.3 mm − 1 (left) and 1.0 mm − 1 (right) patterned illumination. (e) Normalized intensity profiles of the four projection LEDs at their respective detectors. ACL, aspheric condenser lens; DM, dichroic mirror; LP, linear polarizer; AD, achromatic doublet; BPF, bandpass filter; HAT, Hastings achromatic triplet.
    99% Diffuse Reflectance Standard Usrs 99 020, supplied by Labsphere Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Schematic of the combined SFDI and fluorescence imaging system for aPDT dosimetry. (a) Projection arm of the system provides spatially modulated light at four different wavelengths (395, 545, 625, and 850 nm) using two independent amplitude masks. The light is spatially combined and passed through a removable linear polarizer before being projected onto the sample, indicated with an orange arrow, at an angle. (b) Separately, the treatment arm delivers the light to the sample for PDT treatment and fluorescence excitation. The resulting reflectance and fluorescence signals are collected by the imaging arm at three different channels, λ < 590 nm , 590 nm ≤ λ ≤ 660 nm , and λ > 660 , where the middle channel is further cleaned up with a bandpass filter. (c) Picture of the combined imaging system. (d) Pictures of the printed amplitude masks used for 0.3 mm − 1 (left) and 1.0 mm − 1 (right) patterned illumination. (e) Normalized intensity profiles of the four projection LEDs at their respective detectors. ACL, aspheric condenser lens; DM, dichroic mirror; LP, linear polarizer; AD, achromatic doublet; BPF, bandpass filter; HAT, Hastings achromatic triplet.

    Journal: Journal of Biomedical Optics

    Article Title: Development and characterization of a combined fluorescence and spatial frequency domain imaging system for real-time dosimetry of photodynamic therapy

    doi: 10.1117/1.JBO.30.S3.S34103

    Figure Lengend Snippet: Schematic of the combined SFDI and fluorescence imaging system for aPDT dosimetry. (a) Projection arm of the system provides spatially modulated light at four different wavelengths (395, 545, 625, and 850 nm) using two independent amplitude masks. The light is spatially combined and passed through a removable linear polarizer before being projected onto the sample, indicated with an orange arrow, at an angle. (b) Separately, the treatment arm delivers the light to the sample for PDT treatment and fluorescence excitation. The resulting reflectance and fluorescence signals are collected by the imaging arm at three different channels, λ < 590 nm , 590 nm ≤ λ ≤ 660 nm , and λ > 660 , where the middle channel is further cleaned up with a bandpass filter. (c) Picture of the combined imaging system. (d) Pictures of the printed amplitude masks used for 0.3 mm − 1 (left) and 1.0 mm − 1 (right) patterned illumination. (e) Normalized intensity profiles of the four projection LEDs at their respective detectors. ACL, aspheric condenser lens; DM, dichroic mirror; LP, linear polarizer; AD, achromatic doublet; BPF, bandpass filter; HAT, Hastings achromatic triplet.

    Article Snippet: The spectral throughput of the system was assessed by measuring the spectral profiles of each LED at their corresponding detectors [ ] using a miniature spectrometer (Flame-S-VIS-NIR, Ocean Insight, Orlando, Florida, United States), with the light from the illumination arm being reflected off of a 99% diffuse reflectance standard (USRS-99-020, LabSphere, North Sutton, New Hampshire, United States) to allow for collection by the imaging arm without further influencing the spectral response.

    Techniques: Fluorescence, Imaging

    Predicted spatial frequency–dependent reflectance of the human skin. SFDI Monte Carlo simulations were performed using a seven-layer human skin model (a) with a fixed width (1 mm) and depth (3 mm) and a variable length such that two full periods of the patterned illumination were always applied. The thickness ( d , mm), scattering anisotropy ( g ), and refractive index ( n ) for each layer were fixed, whereas the absorption ( μ a , mm − 1 ) and reduced scattering ( μ s ′ , mm − 1 ) coefficients were varied for the four illumination wavelengths. The absolute (b) and normalized (c) Monte Carlo results for the reflectance of the seven-layer model with spatial frequencies between 0 and 1.0 mm − 1 at the four illumination wavelengths.

    Journal: Journal of Biomedical Optics

    Article Title: Development and characterization of a combined fluorescence and spatial frequency domain imaging system for real-time dosimetry of photodynamic therapy

    doi: 10.1117/1.JBO.30.S3.S34103

    Figure Lengend Snippet: Predicted spatial frequency–dependent reflectance of the human skin. SFDI Monte Carlo simulations were performed using a seven-layer human skin model (a) with a fixed width (1 mm) and depth (3 mm) and a variable length such that two full periods of the patterned illumination were always applied. The thickness ( d , mm), scattering anisotropy ( g ), and refractive index ( n ) for each layer were fixed, whereas the absorption ( μ a , mm − 1 ) and reduced scattering ( μ s ′ , mm − 1 ) coefficients were varied for the four illumination wavelengths. The absolute (b) and normalized (c) Monte Carlo results for the reflectance of the seven-layer model with spatial frequencies between 0 and 1.0 mm − 1 at the four illumination wavelengths.

    Article Snippet: The spectral throughput of the system was assessed by measuring the spectral profiles of each LED at their corresponding detectors [ ] using a miniature spectrometer (Flame-S-VIS-NIR, Ocean Insight, Orlando, Florida, United States), with the light from the illumination arm being reflected off of a 99% diffuse reflectance standard (USRS-99-020, LabSphere, North Sutton, New Hampshire, United States) to allow for collection by the imaging arm without further influencing the spectral response.

    Techniques: Refractive Index